Biotin-Histone H3 (14-34) K23Me3 Biotin-KAPRKQLAT-[K(Me)3]-AARKSAPATGG-acid, Where K(Me)3 is Tri-Methylated Lysine

Description

Residues 14-34 of histone 3 (H3) N terminal tail, lysine 23 has been tri-methylated, and N terminal biotinylation of the sequence is present. H3 is one of the four core histones fundamental to eukaryotic DNA compaction into the nucleosome. 

See full description

Catalogue number crb1000343
Sequence (one letter code) Biotin-KAPRKQLAT-[K(Me)3]-AARKSAPATGG-acid, Where K(Me)3 is Tri-Methylated Lysine
Sequence (three letter code) Biotin-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala-Thr-[pThr]-[Lys(Me)3]-Ala-Ala-Arg-Lys-Ser-Ala-Pro-Ala-Thr-Gly-Gly-OH, Where Lys(Me)3 is Tri-Methylated Lysine
Molecular Weight 2376.4
Research Area Epigenetics
Purity >95%
References

Nagarama et al., (2006). Biological functions of biotinylated histones. J. Nutr.Biochem., 7: 446. PMID: 15992689.

 

Henneman et al., (2018). Structure and function of archaeal histones. PLoS Genet., 14(9): e1007582. DOI: https://doi.org/10.1371/journal.pgen.1007582.

 

Su et al., (2016). Reader domain specificity and lysine demethylase-4 family function. Nat. Comms., 7(1): 1. DOI: 10.1038/ncomms13387.

H3 is a core component of the nucleosome, functioning in DNA compaction and availability to transcription machinery. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodelling. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. There is a wealth of data recording these modifications but understanding their significance is not as clear. H3K23me3, an enriched modification in heterochromatin, is known to bind histone demethylase KDM4A. H3K23me3 is also necessary for timely and accurate meiotic divisions.


H3 amino acids 14-34 with lysine 23 trimethylated are provided here with a biotin label for easy use in detection by fluorescence microscopy, ELISA or western blots. Alternatively, it can be purified for protein-protein interactions with the appropriate affinity purification protocol. 

Biotin-Histone H3 (14-34) K23Me3

Cat No. Pack Size Price Qty

crb1000343h

0.5mg

$255.00

crb1000343j

1mg

$330.00