Biotin-Histone H3 (14-34) pT22 K23Me3 Biotin-KAPRKQLA-[pT]-[K(Me)3]-AARKSAPATGG-acid
Histone 3 (H3) is one of the four core histones fundamental for compacting eukaryotic DNA into the nucleosome. The histone tail fragment (14-34) is N terminal biotinylated, lysine 23 has been tri-methylated, and threonine 22 has been phosphorylated (pT22 K23Me3).
Catalogue number crb1000344 Molecular Weight 2456.3 Sequence (one letter code) Biotin-KAPRKQLA-[pT]-[K(Me)3]-AARKSAPATGG-acid Sequence (three letter code) Biotin-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala-[pThr]-[Lys(Me)3]-Ala-Ala-Arg-Lys-Ser-Ala-Pro-Ala-Thr-Gly-Gly-OH Molecular Weight 2456.3 Purity >95% References
Hyland et al., (2005) Insights into the Role of Histone H3 and Histone H4 Core Modifiable Residues in Saccharomyces cerevisiae. Mol. Cell. Bio. 22 10060 PMID: 16260619
Schwartz-Orbach et al., (2020). Caenorhabditis elegans nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3. eLife. 9: e54309. PMID: 32804637
H3 is a core component of the nucleosome, functioning in DNA compaction and availability to transcription machinery. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodelling. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. There is a wealth of data recording these modifications but understanding their significance is not as clear. In Caenorhabditis elegans H3K23me3 can be induced by exogenous dsRNA and this modification can persist for four generations after the dsRNA exposure has been stopped. H3K23me3 is enriched in C. elegans heterochromatic regions, the histone methyltransferase SET-32, methylates H3K23 in vitro.
A 20-mer fragment of the N terminal histone tail is provided here with threonine 22 phosphorylated and lysine 23 tri-methylated (pT22 K23Me3) with an N terminal biotin label attached. The biotin label should allow for easy use in detection by fluorescence microscopy, ELISA or western blots. Alternatively, it can be purified for protein-protein interactions with the appropriate affinity purification protocol.