DYKDDDDK FLAG peptide DYKDDDDK-acid

Description

The FLAG peptide for use in protein purification and localisation

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Catalogue number crb1001213
Sequence (one letter code) DYKDDDDK-acid
Sequence (three letter code) H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH
Molecular Weight 1012.4
Purity >95%
Storage -20°C
References

Stegeman et al., (2016). The spliceosomal protein SF3B5 is a novel component of Drosophila SAGA that functions in gene expression independent of splicing. J Mol Biol. 428(18): 3632. PMID: 27185460

Highly specific protein tag that can be added to a protein using recombinant DNA technology. FLAG is an artificial antigen to which high affinity monoclonal antibodies have been raised, therefore allowing for highly effective protein purification by affinity chromatography as well as accurate localisation of FLAG tagged proteins within living cells, or Western blots. FLAG peptide can be used to effectively purify complexes with multiple proteins as its mild purification procedure tends not to disrupt such complexes. It can be used to obtain proteins of sufficient purity for x-ray crystallography.

DYKDDDDK FLAG peptide

Cat No. Pack Size Price Qty

crb1001213j

1mg

$165.00

crb1001213h

0.5mg

$127.50