Histone H3 (1-20) pT3, K4Me3-GG-[Lys(5-FAM)] AR-[pT]-[K(Me3)]-QTARKSTGGKAPRKQL-GG-[K(5-FAM)]-amide
Histone 3 (H3) is one of the four core histones fundamental for compacting eukaryotic DNA into the nucleosome. Lysine 4 this peptide has been tri-methylated, threonine 3 has been phosphorylated and the C-terminal lysine has been labelled with 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag.
Catalogue number crb1101262 Molecular Weight 2904.5 Sequence (one letter code) AR-[pT]-[K(Me3)]-QTARKSTGGKAPRKQL-GG-[K(5-FAM)]-amide Sequence (three letter code) H-Ala-Arg-[pThr]-[Lys(Me3)]-Gln-Thr-Ala-Arg-Lys-Ser-Thr-Gly-Gly-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Gly-Gly-[Lys(5-FAM)]-NH2 Molecular Weight 2904.5 Purity >95% Storage -20°C References
Henneman et al., (2018). Structure and function of archaeal histones. PLoS Genet. 14(9) e1007582 DOI: https://doi.org/10.1371/journal.pgen.1007582
Stewart et al., (2015). Dynamic changes in histone modifications precede de novo DNA methylation in oocytes. Genes Dev. PMID: 26584620
Nguyen et al., (2014). Phosphorylation of threonine 3 on histone H3 by haspin kinase is required for meiosis I in mouse oocytes. J Cell Sci. PMID: 25315835
Histone 3 (H3) is one of the four core histones (H2A, H2B, H3 and H4) fundamental in compacting eukaryotic DNA into the nucleosome. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. The transcriptional activation or silencing of the chromatin is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes which target histone proteins. Both processes function to alter the positioning of the nucleosome, allowing the DNA it to be either available or inaccessible to the transcription machinery.
The lysine at position 4 of this peptide has been tri-methylated and it is implicated in studies that this modification may remodel the chromatin so that it is more accessible to transcription factors, which may ultimately increase the level of gene expression. Moreover, the threonine at position 3 has been phosphorylated. It is implicated in recent studies that H3T3 phosphorylation regulates chromosome cohesion and is necessary for chromosomes to be able to align on the metaphase plate.
The this peptide is labelled with 5-Carboxyfluorescein (5-FAM), a widely used green fluorescent tag.