Histone H3 (1–21) K4ac ART-[K(Ac)]-QTARKSTGGKAPRKQLA-acid

Description

Histone H3 (1–21) K4ac is derived from Histone 3 (H3) which is one of the four core histones fundamental for compacting eukaryotic DNA into the nucleosome. The lysine 4 has been acetylated.

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Catalogue number crb1000424
Sequence (one letter code) ART-[K(Ac)]-QTARKSTGGKAPRKQLA-acid
Sequence (three letter code) H-Ala-Arg-Thr-Lys(Ac)-Gln-Thr-Ala-Arg-Lys-Ser-Thr-Gly-Gly-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala-OH
Molecular Weight 2295.3
Purity >95%
References

Hyland et al (2005) Insights into the Role of Histone H3 and Histone H4 Core Modifiable Residues in Saccharomyces cerevisiae. Mol. Cell. Bio. (22) 10060 PMID: 16260619

Kothapalli et al (2006) Biological functions of biotinylated histones. J. Nutr. Biochem. (7) 446 PMID: 15992689

Henneman et al (2018) Structure and function of archaeal histones. PLOS. DOI: https://doi.org/10.1371/journal.pgen.1007582

Histone H3 (1–21) K4ac is derived from Histone 3 (H3) which is one of the four core histones (H2A, H2B, H3 and H4) fundamental in compacting eukaryotic DNA into the nucleosome. The nucleosome arises when 147 base pairs of DNA wrap around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. Both H4 and H3 are highly conserved and perform roles in binding to segments of DNA which enter and leave the nucleosome and in chromatin formation. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. Due to histones containing a large number of lysine and arginine residues they have a positive net charge which interacts in an electrostatic manner with the negatively charged phosphate groups in DNA. The transcriptional activation or silencing of the chromatin is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes which target histone proteins. Both processes function to alter to change the positioning of the nucleosome, allowing the DNA it to be either available to the transcription machinery or inaccessible.


The Histone H3 (1-21)’s lysine 4 has been acetylated.

Histone H3 (1–21) K4ac

Cat No. Pack Size Price Qty

crb1000424h

0.5mg

$255.00

crb1000424j

1mg

$330.00