Biotin-Histone H3 (14-34) K23Me3 Biotin-KAPRKQLAT-[K(Me)3]-AARKSAPATGG-acid, Where K(Me)3 is Tri-Methylated Lysine

Description

Histone 3 (H3) is one of the four core histones fundamental for compacting eukaryotic DNA into the nucleosome. The biotinylation of the histone 3 N-terminus is present and lysine 23 has been tri-methylated.

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Catalogue number crb1000343
Sequence (one letter code) Biotin-KAPRKQLAT-[K(Me)3]-AARKSAPATGG-acid, Where K(Me)3 is Tri-Methylated Lysine
Sequence (three letter code) Biotin-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala-Thr-[pThr]-[Lys(Me)3]-Ala-Ala-Arg-Lys-Ser-Ala-Pro-Ala-Thr-Gly-Gly-OH, Where Lys(Me)3 is Tri-Methylated Lysine
Purity >95%
Molecular Weight 2376.4
References

Hyland et al., (2005). Insights into the Role of Histone H3 and Histone H4 Core Modifiable Residues in Saccharomyces cerevisiae. Mol. Cell. Bio. 22 10060 PMID: 16260619

 

Nagarama Kothapalli et al., (2006). Biological functions of biotinylated histones. J. Nutr. Biochem. (7) 446 PMID: 15992689

 

Henneman et al., (2018). Structure and function of archaeal histones. PLoS Genet. 14(9) e1007582 DOI: https://doi.org/10.1371/journal.pgen.1007582

Histone 3 (H3) is one of the four core histones (H2A, H2B, H3 and H4) fundamental in compacting eukaryotic DNA into the nucleosome. The nucleosome arises when DNA wraps around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. Both H4 and H3 are highly conserved and perform roles in binding to segments of DNA which enter and leave the nucleosome and in chromatin formation. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. The transcriptional activation or silencing of the chromatin is controlled by chromatin remodelling factors and histone modifying enzymes which target histone proteins. Both processes function to alter the positioning of the nucleosome, allowing the DNA it to be either available or inaccessible to the transcription machinery.

H3K23me3 has been shown to be associated with heterochromatin and is necessary for timely and accurate meiotic divisions.

Biotin-Histone H3 (14-34) K23Me3

Cat No. Pack Size Price Qty

crb1000343h

0.5mg

$255.00

crb1000343j

1mg

$330.00