Biotin-Histone H3 (14-34) pT22 K23Me3 Biotin-KAPRKQLA-[pT]-[K(Me)3]-AARKSAPATGG-acid


Histone 3 (H3) is one of the four core histones fundamental for compacting eukaryotic DNA into the nucleosome. The biotinylation of the histone 3 N-terminus is present, lysine 23 has been tri-methylated, and threonine 22 has been phosphorylated.

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Catalogue number crb1000344
Sequence (one letter code) Biotin-KAPRKQLA-[pT]-[K(Me)3]-AARKSAPATGG-acid
Sequence (three letter code) Biotin-Lys-Ala-Pro-Arg-Lys-Gln-Leu-Ala-[pThr]-[Lys(Me)3]-Ala-Ala-Arg-Lys-Ser-Ala-Pro-Ala-Thr-Gly-Gly-OH
Molecular Weight 2456.3
Purity >95%

Hyland et al., (2005) Insights into the Role of Histone H3 and Histone H4 Core Modifiable Residues in Saccharomyces cerevisiae. Mol. Cell. Bio. 22 10060 PMID: 16260619


Schwartz-Orbach et al., (2020). Caenorhabditis elegans nuclear RNAi factor SET-32 deposits the transgenerational histone modification, H3K23me3. eLife. 9: e54309. PMID: 32804637


Histone 3 (H3) is one of the four core histones (H2A, H2B, H3 and H4) fundamental in compacting eukaryotic DNA into the nucleosome. The nucleosome arises when 147 base pairs of DNA wrap around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain, the “histone tail” which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. The transcriptional activation or silencing of the chromatin is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes which target histone proteins. Both processes function to alter the positioning of the nucleosome, allowing the DNA it to be either available or inaccessible to the transcription machinery.

Lysine 23 has been tri-methylated and threonine 22 has been phosphorylated. In C.elegans H3K23me3 can be induced by exogenous dsRNA and this modification can persist for four generations after the dsRNA exposure has been stopped. H3K23me3 is enriched in C. elegans heterochromatic regions, the histone methyltransferase SET-32, methylates H3K23 in vitro.

Biotin-Histone H3 (14-34) pT22 K23Me3

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