Tetrazine reagents

Tetrazines are a type of click chemistry labelling reagents that react with TCO very rapidly and selectively in inverse-electron demand Diels-Alder reactions (IEDDA), producing conjugates. The water-tolerant IEDDA reaction is then followed by a retro-DA reaction to eliminate Nitrogen gas. They are useful for bioconjugations such as, labelling live cells and molecular imaging.